KMID : 1140420180270020102
|
|
Journal of Obesity & Metabolic Syndrome 2018 Volume.27 No. 2 p.102 ~ p.109
|
|
Quercetin Upregulates Uncoupling Protein 1 in White/Brown Adipose Tissues through Sympathetic Stimulation
|
|
Choi Hyun-Jung
Kim Chu-Sook Yu Ri-Na
|
|
Abstract
|
|
|
Background: Uncoupling protein 1 (UCP1) plays an important role in increasing energy expenditure; thus, it is being considered as a new target for preventing obesity and metabolic complications. In this study, we investigated the effect of quercetin, a naturally occurring flavonoid, on UCP1 expression in white/brown adipose tissues (WAT/BAT).
Methods: Mice were fed a high-fat diet (HFD) supplemented with or without dietary quercetin for 9 weeks, and 3T3-L1 adipocytes were treated with quercetin. Expression of UCP1 and other thermogenic genes/proteins was measured by real-time polymerase chain reaction and/or Western blotting.
Results: Dietary quercetin supplementation increased the level of UCP1 in both WAT and/or BAT of HFD-fed obese mice, which was accompanied by upregulated mRNA levels of thermogenesis-related genes. Quercetin supplementation enhanced the plasma norepinephrine level and tended to upregulate ¥â-adrenergic receptor mRNA level in the WAT of HFD-fed obese mice, accompanied by AMP-activated protein kinase (AMPK) activation. Moreover, quercetin enhanced UCP1 expression in 3T3-L1 adipocytes, and this was blunted by treatment with a peroxisome proliferator-activated receptor gamma (PPAR¥ã) antagonist.
Conclusion: These findings suggest that quercetin upregulates UCP1, implying increased WAT browning and BAT activity, via activation of the AMPK/PPAR¥ã pathway through sympathetic stimulation. Quercetin may be useful for preventing obesity and metabolic complications.
|
|
KEYWORD
|
|
Obesity, Adipose tissue, Uncoupling protein 1, Quercetin
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|